We did not explore lower pore-size gels, as a 150 kDa antibody is ∼30 nm in size and gel pore sizes of ∼20 nm are expected in 10.5%T, 5%C PA gels.
These efforts included digestion of samples with proteinase K, DNAse and RNAse treatments, ultracentrifugation, gel filtration, and extensive dialysis that are expected to remove low-MW compounds and retain large CPS polymers.
Functional fiber characteristics, such as water solubility, viscosity, gelling, water binding capacity, and fermentability, are expected to be pivotal in the regulation of satiety (e.g., Papathanasopoulos and Camilleri, 2010).
The determination of the remaining film thickness by means of FTIR spectroscopy was not applicable in this case, because the glycosidic bonds which were used for the previous sol gel analysis are expected to be hydrolyzed due to enzyme activity.
However, in contrast to DIGE where the Cy2-labelled sample is formed by pooling all samples and used as internal standard, our experiment had to be designed slightly differently to accommodate the fact that Cy2 labelling results in gels 1 3 were expected to differ from those in gels 4 6 due to different quencher concentration.
Therefore, these gels could be expected to maintain structure in vivo due to the presence of a highly elastic structure and an ability to imbibe fluid with a limited effect on the rheological structure.
So the decreased viscosity of the gel system is expected to improve the injectivity, which can contribute to the conformance improvement of deep reservoirs.
In this way, when solid dispersions are placed in water at 37°C, gel formation is expected [ 13– 15] which may affect the kinetic release mainly due to the increase in viscosity.
The Follow on Africa Consortium for Tenofovir Studies FACTSS) 001 trial is repeating the CAPRISA 004 trial design in broader populations in South Africa evaluating pericoital use of tenofovir gel and is expected to report results in 2013 (FACTS, 2012).
