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Gel transfers were performed in a Hoeffer wire transfer tank at 50 volts for 2 hours at 4°C.
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Total proteins were separated on a 12% SDS/PAGE gel, transferred to nitrocellulose membrane (Whatman).
The DNA/protein mixtures were then analyzed by the native 7% PAGE, and directly transferred onto nylon membrane by contact blotting-aided gel transfer.
Following electrophoresis, proteins in the gel were transferred onto nitrocellulose membranes (iBlot Gel Transfer Stacks, Invitrogen).
Protein lysates were resolved on denaturing 8 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (iBlot Gel transfer stacks, Invitrogen).
The proteins were transferred to nitrocellulose membrane using iBlot™ gel transfer device (Invitrogen).
Proteins were transferred to a nitrocellulose membrane using iBlot Gel Transfer Device (Invitrogen).
Proteins were transferred to a nitrocellulose membrane using the iBlot gel transfer system (Invitrogen).
Samples were analyzed by SDS-polyacrylamide gel, transferred to nitrocellulose membranes and autoradiographed.
For immunoblotting, protein samples were separated on a 6% SDS-PAGE gel, transferred to Immobilon™ Transfer Membrane (Millipore, Bedford, MA).
For immunoblotting, the separated proteins were electrophoretically transferred to a nitrocellulose membrane using an iBlot gel transfer system (Invitrogen).
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