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The gel was then submerged in a gel tank containing TAE buffer, pH 5 and a current of 35 mV passed across the gel (Figure 3B).
Gel tray was placed in gel tank containing 1000 mL 0.5X TBE buffer.
Molecular grade agarose gel and Tris-Borate EDTA buffer used in the gel tank were freshly prepared.
To prevent heating of the gel and subsequent denaturing of the DNA duplex the gel tank was placed in a plastic container containing an ice and water mixture.
10 16 Gel tank, gel comb and excision blade were cleaned with DNA-away spray and sterilised by exposing to UV for 30 min.
Normal and tumour samples from the same patient were run in the same gel tank to account for any differences caused by gel electrophoresis.
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So instead of basking in the warm San Francisco sunshine, I spent a miserable fortnight trying not to drop bits of salad in the gel tanks by accident.
In this step, the slides are placed first in a horizontal gel electrophoresis tank and then tank pour with fresh and chilled electrophoresis solution (1 mM EDTA and 300 mM NaOH, pH > 13).
Up to 160 samples/day if using multi-gel (8 gels) buffer tank.
The slides were then removed from the lysis solution and placed on a horizontal gel electrophoresis tank.
The slides were then removed from the lysis solution and placed in a horizontal gel electrophoresis tank.
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