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The semiquantitative RT-PCR images were evaluated and pixels in respective bands were quantified using IPLab gel software for a 12 bit image.
Relative optical density of protein bands was analyzed using gel software image lab 3.0.
Quantification of signals was carried out by Un-Scan-It gel software (Silk Scientific, UT, USA).
Bands were quantified using the UN-SCAN-IT gel software (Silk Scientific, USA) and normalized to a loading control (Cyclophilin B, Abcam).
Expression of eNOS was quantified using the UN-SCAN-IT gel software (Silk Scientific, Orem, UT, USA) and normalized to β-actin content.
Bands were quantified using UN-SCAN-IT gel software (Silk Scientific, USA), and all samples were run in duplicate on separate gels to confirm results.
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Densitometric analysis of the results was performed using the EZQuant-Gel software and the percentage of topo II activity was calculated.
Densitometric analysis of the results was performed using the EZQuant-Gel software and the percentage of topo I activity was calculated.
The Gel Compar software package (version 4.0; Applied Maths, Bionumerics) was used to compare the band patterns.
Analysis of the 2D fluorescent images was performed using the DeCyder™ BVA module version 6.5 (GE Healthcare), a 2D gel analysis software package designed specifically for DIGE.
After gel imaging, software analysis revealed protein spots differentially expressed between the 2 groups; these spots were excised and identified by mass spectrometry.
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