Sentence examples for gel selection from inspiring English sources

For Solexa sequencing, ChIPed DNA was prepared according to the Illumina protocol with two modifications: (1) DNA fragments ranging from 150 to 300 bp were selected at the gel selection step and (2) 21 instead of 18 cycles of PCR were done at the amplification step, as previously described (Cao et al, 2010).

When the digested genomic DNA underwent gel selection, the portion of the digested DNA that can be finally selected by the selection section window (40-300 bp) will be changed.

The fragmented DNA was further subject to end repair, addition of "A" bases, ligation of NGS adaptors, gel selection of 300 bp fragments and PCR enrichment according to the protocol provided by Yale Center for Genome Analysis (YCGA, http://ycga.yale.edu/sequencing/Illumina/protocols.aspx) with reagents ordered from NEB.

We used the same reference genome (GRCh37) that was used for RRBS mapping as the virtual test genome, subjecting it to MspI digestion and recovery of the resulting DNA from the gel selection by keeping the proper size of the digested DNA fragments in the in-silico stimulation.

Twenty five microliters of immunoprecipitated or input DNA (ranging from <1 ng – 4 ng DNA) was used for library preparation using Illumina (San Diego, CA) TruSeq™ DNA Sample Preparation reagents, with ligation of 1/10th the manufacturers recommended adapter amounts and agarose gel selection of DNA fragments in the 200 500 bp size range.

A single agarose gel size selection step was performed (selecting DNA from 500-800bp) prior to PCR amplification of 5-8 cycles depending on input DNA (estimated using the Agilant 2100 Bioanalyser).

DNA was fragmented using Covaris technology and the libraries were prepared without gel size selection.

Gel size selection of small RNAs and subsequent sequencing library construction were completed as described (Lu et al. 2007).

For the Illumina Paired-end Sequencing protocol the fragment size of 300-450 bp was reached by agarose gel size selection.

The only departure from the protocol concerned the step of agarose gel size selection, which was skipped.

Briefly, 4 μg of genomic DNA was fragmented per manufacturer's protocol followed by strand displacement, agarose gel size selection of 5-kb target size, and circularization.