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Because binding is noncovalent, such a reagent could be used in a displacement assay performed in a native gel, monitoring decrease in fluorescent band intensity.
Fractions (1 ml) were eluted from the bottom of the gel, monitoring at 280 nm.
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Figure 5A shows SDS PAGE gels monitoring the reconstitution process.
(A ) Native gels monitoring yU4/U6 di-snRNA unwinding by yBrr2 in the absence of other proteins (top), in the presence of yPrp3CTF (middle) or yPrp3DUF1115 (bottom).
(D ) Imaging of nonreducing SDS-PAGE gels monitoring pulse-chase fluorescent Ub transfer from E2 to Rsp5 to substrate for the indicated versions of Rsp5, in the absence or presence of Sna3C.
(B ) Fluorescent imaging of reducing SDS-PAGE gels monitoring multiple turnover fluorescent Ub ligation by full-length Rsp5 (Rsp5FL), or a construct containing all three WW domains plus the HECT domain (Rsp5WW1-3-HECT), in the presence of wild-type or a P107A PPXY motif mutant version of Sna3C.
Stain-free gels (Bio-Rad) contain a compound evenly distributed in the precast acrylamide gel that reacts with tryptophan following UV exposure and gives a strong fluorescent signal that can be used to stain for total-protein levels in acrylamide gels, monitor transfer in immunoblotting, and serve as a loading control.
To achieve this goal, aspirates were embedded in a fibrin gel and monitored for their stability in dynamic and static culture conditions over a period of 28 days (Madry et al. 2013).
To study the relationship between cell adhesion forces and network structure, cells were cultured on fibrin gels coated with fluorescent beads, and the contraction or relaxation of the gel was monitored by tracking bead displacements in response to drug treatments.
An aliquot (3 µl) was examined on a 1% TAE agarose gel to monitor amplification.
Optically distinguishable foreign material was added to the gel and monitored by confocal microscopy.
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