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Quality and quantity of DNA was monitored by spectrophotometry and gel inspection.
After manual gel inspection of spot alignment, the image analysis annotated a total of 597 spots.
Although similar AP activity was loaded on to the IEF gel, inspection revealed notable differences in the band intensities.
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Purified reaction products were run out on a 2%% agarose gel for visual inspection and quantified using the Qubit dsDNA High Sensitivity fluorometric assay (Invitrogen).
Four microliters of PCR product was run on a 1% agarose gel for visual inspection of size, and 26 μL of the remaining PCR product was transferred to a flat bottom 96-well PCR plate (VWR).
The PCR products were loaded on 2% agarose gels for visual inspection of band size.
RNA pellets were resuspended in 20 μl of warm diethyl pyrocarbonate-treated water, vortexing gently for about 15 min. RNA samples were quantified in a spectrophotometer and loaded on 1% agarose/formaldehyde gels for quality inspection.
The isolated plasmid preparations were analysed by agarose gel electrophoresis for visual inspection.
Thus, final library selection is determined by visual inspection of gel images for appropriate smearing and satellite band patterns.
All RNA samples were analyzed by formaldehyde agarose gel electrophoresis and visual inspection of the ribosomal RNA bands upon ethidium bromide staining.
Amplification products were detected by visual inspection, agarose gel electrophoresis and by real-time monitoring of turbidity resulted by formation of LAMP amplicons.
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