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(b) Gel filtration chromatography.
L.S. provided technical support for the gel filtration.
Gel filtration steps are same as the above.
b, SDS PAGE analysis of the complex after gel filtration.
The column was calibrated with Bio-Rad gel filtration standards.
g, Analysis of the γ-secretase Notch-100 complex by gel filtration.
Gel filtration profiles also indicated that AcrIIA6 is a dimer in solution (Supplementary Fig. 3).
An extended PML sequence, RING1 119 was thus subjected to gel filtration and SEC-MALS analysis.
By gel filtration chromatography, the molecular mass of the enzyme was estimated to be 130 kDa.
The enzyme substrate complex was purified by affinity chromatography and gel filtration (see Methods).
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a, Representative gel-filtration chromatography of NavAb(R3G); highlighted peak fractions were concentrated for crystallization.
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