Sentence examples for gel detection from inspiring English sources

Large multiplex ARMS containing more primer mixes are feasible, increase the number of mutations detected, but agarose gel detection of PCR limits the useful size of the multiplex.

Alternatively UL37 spliced variants are exceedingly low abundances relative to the UL37x1 unspliced transcript, some ~100 fold less and below detection by RT-PCR and gel detection, so the alternatively UL37 spliced variants cannot be detected by either S1 or Northern blot analysis [ 17, 18].

Based on this indel polymorphism, a pair of specific primers was designed and used to develop a specific-PCR polyacrylamide gel detection assay.

Cohen, HJ. "The use of diaminobenzidine for spectrophotometric and acrylamide gel detection of sulfite oxidase and its applicability to hydrogen peroxide-generating enzymes". Analytical Biochemistry 53, no. 1 (1973): 208-222.

Next, TCR beta clonality was measured using the In Vivo Scribe TCRB Gene Clonality Assay for gel detection, according to manufacturer's instructions (San Diego, CA).

Endpoint agarose gel detection showed the expected TSP genotyping products in assays configured for both allele-specific PCR and HRM detection.

BGAL4-TEV-IFP-6xHis was the only protein that could not be detected by in-gel detection.

In-gel detection of LDH activity was using a tetrazolium-salt-based chemistry (CytoTox 96®, Promega).

Time-dependent changes of infrared signal intensity were monitored by in-gel detection after SDS-PAGE of TEV-treated proteins.

After 1 h, 2 h and 4 h of incubation 40-µL aliquots were separated by SDS-PAGE analyzedyzed by in-gel detection followed by Coomassie staining.

Samples (10 20 µL) were separated by SDS-PAGE and analysed by in-gel detection or western blot and infrared analysis as described [21].