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The raw gel data from the conventional PCR are shown in Supplementary Fig. S4A.
This file contains Supplementary Figures which show source gel data scans and a Supplementary Table.
The SCVs were also randomly checked for absence of plasmid by agarose gel (data not shown).
The gel conversion was calculated by correlation of the rheological gel data to d.s.c.s.c
Both C. minus verum and simile produced the expected 390 bp amplicon on an agarose gel (data not shown).
Electrophoresis gel data from the imaging systems were visualized and analyzed with Quantity One 1-D analysis software.
The DTT displayed the gel data from SQL*LIMS™ corresponding to the identification numbers in the 2-DE database (Figure 3B).
The purity of the GST-Rab3D recombinant protein was confirmed on a Coomassie Blue stained SDS-PAGE gel (data not shown).
Another P. antarctica strain, T-34, also produced xylanase (18.5 U/ml) under the same conditions with xylose, and the 33-kDa unknown protein band was also detected on SDS-PAGE gel (data not shown).
The membrane proteins responsible for nanoparticle synthesis were run along with β-met-treated membrane proteins in SDS-PAGE gel (data not shown) which confirmed the presence of different sizes of protein bands in the reaction mixture, of which 25 and 73 KDa seemed to be of importance.
Sixty-four samples (7.7%) were positive for HRV, according to the length of the amplified fragment in the VP4/VP2 region visualized on agarose gel (data not shown).
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