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(B ) Compared to wild-type, β T238A αβ-tubulin is better able to populate a straight conformation on the end of GDP containing microtubules.
All subjects will undergo a 2-month run-in period, in which they receive conventional lactate buffered, acidic, GDP containing PD fluid.
Rats in the groups treated with GDPs, such as methylglyoxal (MGO) or formaldehyde (FA), intraperitoneally received 100 mL/kg/day GDP containing PD fluids (PDFs) for 21 days.
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RAB-5(GDP) contains an S23N substitution, locking it in the GDP-bound conformation and depressing endocytosis.
Samples with GDP contained 1.5 mM GDP and 5 mM MgCl2.
This concept is supported by a short-term European study which reported greater urine volume and both urinary urea and creatinine clearance with the neutral pH low GDP glucose containing dialysates [ 66].
The revised second-quarter GDP data contain a record-setter: Businesses reduced their investment spending for the seventh consecutive quarter, the longest string of negative quarters since 1947.
We generated a transgene, Pglr-1::rfp::rab-5(GDP), which contains a mutated form of the rab-5 cDNA expressed from the glr-1 promoter.
We had previously generated a transgene, Pglr-1::rfp::rab-5(GDP), which contains a mutated form of the rab-5 cDNA that can suppress the clathrin-dependent endocytosis of GLR-1 [56].
For measurements in the presence of GDP, all proteins were dialysed beforehand against buffer containing GDP, and the measurements were performed in this buffer to counteract any effects of nucleotide binding.
However, the stronger binding of Bim1 to GDP-containing β T238A microtubules indicates that the mutant microtubule lattice retains more GTP-like character than does wild-type.
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