Exact(8)
That decline, which was a bit worse than expected, left GDP 1% lower than a year ago.
Briefly, 20 µg purified early endosomes were incubated for 20 min at 30°C in EEB (75 mM K-Acetate, 30 mM HEPES pH 7.4, 5 mM MgCl2) containing 0.3 mM ATP, 3 mM GDP, 1 µM GST-GDI and 100 µg cytosol.
Binding buffer contained 50 mM Tris/HCl, pH 7.4, 2 mM triethanolamine, 1 mM EDTA, 5 mM MgCI2, 10 µM GDP, 1 mM dithiothreitol, 100 mM NaCl, 0.2 units/ml adenosine deaminase, 0.2 nM [35S]GTPγS, and 0.5% bovine serum albumin.
Costs were converted to 2013 values using the Malawi consumer price index and to international dollars using the implied 2013 purchasing-power parity conversion factor for GDP (1 INT$ = 105.8 Malawi Kwacha) [ 14].
Total cell lysates were prepared in ice-cold lysis buffer (30 mM HEPES, 100 mM KCl, 20 mM NaCl, 2 mM MgCl2, 5% glycerol, 2% heptanetriol, 0.5% laurylmaltoside, 5 μM GDP, 1 μM microcystine, 1 mM sodium orthovanadate and a complete mixture of protease inhibitors (Roche, Meylan, France)).
Purified Rab and HRas proteins (4 µM or 6 µM final for each) were incubated at 30°C for 2 hours in HN150 containing MgCl2 (6 mM), DTT (1 mM), and GTP (1 mM), GDP (1 mM), or GTPγS (1 mM) where indicated.
Similar(51)
As a proportion of GDP, 80.3% this year, 79.1% the following year to reach 68.5% in 2020/21.
Fabrizio Goria (@FGoria) Czech GDP (Q4 A) Q/Q 1.6% vs. Exp.
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