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Of 47 patients divided according to their GCS rating, 34 had a GCS ≤ 7 and 13 had GCS > 7.
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Along the D. melanogaster lineage, we identify 207 preferred biased loci (fig. 1; 102 faster GC rate only and 105 slower AT rate only) and 390 unpreferred biased loci (fig. 1; 372 faster AT rate only and 18 slower GC rate only).
Both the GC rate and AT rate were compared between synonymous sites and intron sites at each locus individually.
In particular, our chromosome-wide estimate of GC rate is close to that estimated from the rosy locus.
Genome rearrangements were shown to severely confound identification of genuine NCO GCs, but stringent filters led to highly similar GC rate estimates for both offspring types.
Yang et al. (2012) investigated NCO GCs through whole-genome sequencing of 40 F2 offspring, reporting a 130-fold higher GC rate than Lu et al. (2012).
We make gene-by-gene comparisons between paired synonymous sites (2-fold and 4-fold degenerate) and intron in what we term GC rate and AT rate.
For most loci, there is no detectable difference between such sites in what we term GC rate or AT rate (82% D. sechellia, 86% D. melanogaster).
Using this ancestral sequence, we calculate what we term "GC rate" and "AT rate" at synonymous sites and intron sites, separately.
gDNA fluorescence variability was analysed (ANOVA F-test and regression models) regarding the following parameters: probe GC rate, probe size, probe specificity, putative hairpin and homoduplex entropies (ΔG).
Sun et al. (2012) estimated a GC rate of 3.5 × 10−4 per marker per meiosis based on fluorescent reporter genes in pollen tetrads (Francis et al., 2007).
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