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We have designed four siRNAs for the human GCS gene and transfected them into HeLa cells.
We present a new approach based on the inhibition of the GCS gene using siRNAs as a potential therapeutic strategy for Gaucher disease.
MBO-asGCS has been designed to target the exon-1 of human GCS gene [23], [29].
Effectively silencing the GCS gene in vivo can determine whether GCS has a role in tumor drug resistance.
As shown in Fig. 1b, MBO-asGCS, not MBO-SC, decreased GCS gene expression as measured by mRNA and protein levels, in a dose-dependent manner.
Aimed to determine the role of GCS in tumor response to chemotherapy, a new mixed-backbone oligonucleotide (MBO-asGCS) with higher stability and efficiency has been generated to silence human GCS gene.
Similar(52)
A stilbene dioxygenase gene, (sdoG) is incompletely but broadly conserved in GC gene clusters.
Certain polymorphisms in the GC gene have previously been associated to COPD [ 28, 29].
We will refer to this as a putative gentisate catabolism (GC) gene cluster.
DBP is encoded by the single copy GC gene (NCBI GENE ID2638) located on chromosome 4q12-q13.
A similar effort of normalization at the level of individual words is required for effect, GC, gene and TF.
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