Sentence examples for gcc act from inspiring English sources

A fragment encompassing the INPP5D 3'UTR was PCR-amplified using the forward primer (5'->AGC CCT CAG TGA GCT GCC ACT GAG TCG ->3') and reverse primer (5'->GAG TGA GAA AGG CAC AAT TTA ATT GG->3').

For argT following primers were used: forward, 5'-acc gtg ata gtt ccc cag cgc ggc gcg tta tcc cct tcc cgt gta ggc tgg agc tgc t-3' and reverse, 5'-cac aca acg cca cgt aaa aca taa gaa aat gac gcc act tca tat gaa tat cct cct tag-3'.

The CD98-pcDNA3.1/V5-His-TOPO S305A-S307A-S309A mutant was generated by changing the codon at 1440 1442*, 1446 1448* and 1452 1454* position from serine 305, 307 and 309, respectively, to alanines using specific primers P1 (forward, 5'-C TCA TAC CTG GCT GAT GCT GGC GCC ACT GGG GAG C-3'; reverse, 5'-G CTC CCC AGT GGC GCC AGC ATC AGC CAG GTA TGA G-3').

The PCR primers were as follow: GAPDH: sense: 5'-GTG GGC CAT GAG GTC Cantisensetisense: 5'-TCC ATG ACA ACT TTG GTA TCG T-3' (478 bp); MIF: sense: 5'-TCA CCG CCT CGG CTT GTC antisenseisense: 5'-ATG AAC TTT CTG CTG TCT TG-3' (198 bp); GRK2: sense:5'-TTC TCG AAG AGT GCC ACT G-3', antisense: 5'-CAT TCA TGG TCA GGT GGA TG-3' (202 bp).

The R145W and I175T variants were sequenced from a 409 bp amplicon generated using the following primers: 5'-TTG CCT TCT TAG GCT ATT TTC C-3' and 5'-AAA GGT TCC ATT GCC ACT GT-3'.

PCR was run using 1 μg DNA with the two following primers – ORF1: H1for (285 5' AAC TGT AAA ATG GAT ACG AAC A 306306), H1rev (1808 5' GCC ACT TCA TAA ATT GTT CC 3' 1827).

The primers were as follows [ 16]: Se5′-TTT5′-TTT CAC TGC CAC CAG CAC CTG-3′; Se385′-ATC5′-AAA AAA GGC ACT GGG ACC CAG-3′; Se385′-GGA5′-GGA CGT ACT CCC CCG GGA T-3′; Se385′-TGG5′-TGG AGG AGG AAT ACC GCC ACT-3′; Se385′-GTC5′-GTCCCCC TCG GCG AAC ATG G-3′.

The TNF promoter was amplified using the following primer sets: for human, apes, and OWM, TNF-F1 (5'-AGT GAG AAC TTC CCA GTC TAT CTA and3') and TNF-R1 (5'-CCG TGG GTC AGT ATG TGA GA-3') [19]; for NWM and lemur, TNF-F2 (5'-CCC AAT AAA CCT CTT TTC TCT GA-3'), TNF-F3 (5'-TTG GAA GCC AAG ACT GAA and-3') and TNF-R2 (5'-GTG CCA ACA ACT GCC TTT A-3').

The eluted genomic DNA was subjected to SYBR green real-time qPCR with the APAF1 ChIP primers, forward GCC CCG ACT TCT TCC GGC TCT TCA and reverse GGA GCT GGC AGC TGA AAG ACT C. The results were expressed as fold enrichment over control (NT siRNA or pLPC-Flag).

Human PIK3CA forward primer: TCA AAG GAT TGG GCA CTT TT, and reverse primer: GCC TCG ACT TGC CTA TTC AG.

Genotyping was performed by PCR using genomic DNA and primers specific for Cldn6 (5'-ATG GCC TCT ACT GGT CTG Cand') and the FLAG® tag (5'-TCA CTT GTC ATC GTC GTC CTT G-3').