The interstitial cortisol concentration represents the available GC pool, which is able to enter the cell and bind to the GC receptor.
To discover plasma membrane protein alterations associated with GC, six pools of plasma membrane samples (three controls and three GC) were generated by pooling samples from 4 subjects for each pool.
Pooled GC RNA from 10 patients was analyzed for gene expression profile using Agilent gene chip array.
The database origin of cDNA pools does not affect our coverage optimization after controlling for differences in sequence length, total pool size, GC content and the proportion of ambiguous nucleotides (see additional file 4: "Influence of database origin on pool coverage").
To increase the coverage of protein identifications and the confidence of the data generated, three pools of adjacent normal tissues were labeled with TMT reagents 126, 127 and 128 respectively; pools of GC tissues were labeled with TMT reagents 129, 130 and 131 respectively.
The subsequent data are used to extract the effect of different GC composition and dNTP pools.
Differential miRNAs were identified in serum pools of GC and control and validated gastric cancer and dysplasia versus controls pairs, respectively.
Trinucleotide SSRs were pooled by GC content categories (i.e. 0%, 33%, 67%, or 100%) to increase sample size and no significant effects on allelic richness were detected; however, this likely reflects small within-group sample size (< 8 in most cases) (Table 4).
(A ) Hexane extracts from pooled leaf samples of field-grown plants for a qualitative assessment of green leaf volatile (GLV) pools, analyzed by GC-MS with a split ratio of 1/100 onto a nonpolar column; only (E )-hex-2-enal was identofied due to poor resolution of (E )-hex-2-enal and (Z )-hex-3-en-1-on on the nonpolar column; no ester peaks were detected.
The scaffolds were pooled in bins of 1% GC, in order to analyze the GC profile.
Aspirates from two to five patients were pooled for GCs preparation, and GCs were purified according to a method described.
