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Figure 1A shows an agarose gel of genomic DNA isolated from A. thaliana and G. aurea with Protocol A. Protocol B was attempted in species that gave difficulties in DNA isolation with Protocol A and also in species where large amounts of tissue were available, as the protocol requires 10 g of starting material.
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These features give difficulties when the breeding gain (BG) of the MSR is evaluated.
However, there have been concerns about the production of long-lived 26Al that could give difficulties in the disposal of material following decommissioning.
It is important to include healthy women in trials, given difficulties in recruiting and retaining women in clinical trials [27].
Given difficulties in using available museum collections, it will be necessary to acquire fresh material, especially for **** and *** samples.
It was viewed that "a good man was hard to find" given difficulties that men had with fidelity or commitment as well as shortages of available men.
Too many yeast cells in a tranformation mix will significantly drop the efficiency and will give difficulties to spread on the plates.
No urine output data have been collected in SIRS, given difficulties with its accurate measurement in the setting of international data collection.
Given difficulties in measuring lycopene from FFQs (Kristal and Cohen, 2000), we used the frequency of reported consumption of tomatoes and products with high tomato content.
Each block must have the proof of work of the given difficulty if it is to validated in consensus.
A minority of perplexing specimens still give difficulty in deciding their identity.
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CEO of Professional Science Editing for Scientists @ prosciediting.com