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RP-HPLC analysis of culture supernatants gave concentrations of 149 and 213 mg rSAK-2/L for FEED_I and FEED_II, respectively.
Replicate analyses of this SRM using the method described above gave concentrations of 82.7 ± 1.7 μg/L, which is in good agreement with the certified value.
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Serial dilutions of stock solutions were prepared to give concentrations of 8 90, 20 100 and 20 200 µg/mL for CET, PPA and NMS respectively.
LN cells (5×105 cells/well) were cultured with given concentrations of MOG35 55 for 3 days, with the last 18 h in the presence of 0.5 mCi [3H]thymidine.
There was no inhibitory effect of ethanol on all tested microorganisms at given concentrations of the solvent.
Nutrients were added to the fermenter to give concentrations of 0.5 g/l (NH4 2HPO4 and 0.025 g/l MgSO4*7H2O.
The test sample was then prepared through serial dilution of the stock solution to give concentrations of 4 μM, 38 μM, 96 μM, 192 μM, and 384 μM.
In this model, a linear equation connects microbiota changes to given concentrations of each of the four dietary ingredients (Casein, Starch, Sucrose, and oil).
2-AB and NaBH3CN were reconstituted in DMSO/acetic acid (7 3, v/v) to give concentrations of 0.35 and 1 M, respectively.
Subconfluent H4IIE cells were cultured with the control group (DMSO) and the given concentrations of isoquercitrin (50, 100, or 200 μM) in DMEM for 24 h.
Cells were allowed to grow for 14 days after which cytotoxicity testing was undertaken when cells were exposed to given concentrations of chemical for 24 h.
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CEO of Professional Science Editing for Scientists @ prosciediting.com