Sentence examples for gauging transfection efficiency from inspiring English sources
copGFP Control Plasmid (cat # sc-108083) is a transfection-ready lentiviral vector plasmid that encodes copGFP fluorescent protein in mammalian cells, and used to monitor delivery of shRNA lentiviral construct into cells thereby gauging transfection efficiency.
The plasmid pCMV-Nanog (Origene) is used to overexpress Nanog, alternatively pmaxGFP (Lonza) is used to express GFP as stuffer DNA to gauge transfection efficiency.
At 24 h after transfection, cells underwent RT-qPCR and Western blot to verify transfection efficiency.
The transfection efficiency was confirmed by Western blot (Fig. S2).
The transfection efficiency was investigated by flow cytometry.
Transfection efficiency was normalized to β-Gal activity.
Results showed that it was proportional to cell transfection efficiency.
Figure 4 In vitro transfection efficiency of EGFP-loaded NPs.
The prepared nano-complex formulation achieved high gene transfection efficiency.
Renilla was used as an internal control for transfection efficiency.
The transfection efficiency was presented in Fig. 2A.
