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Percent identity matrices were determined by above methods to gauge the sequence identity of the other old members with respect to the new members.
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To gauge the extent of mls sequence homology among different mycolactone-producing mycobacteria (MPM) we took advantage of a single nucleotide polymorphism (SNP) identified within the KS domain during sequencing of pMUM002.
Some of the following additional issues could bring clarity to such an analysis: 1) ChIP-chip does not identify the precise location of the binding of Mediator components on the genome sequence, to gauge the location of the Mediator complex (and thus the sites from which potential small RNA are generated) ChIP-seq experiments would be of considerable value.
24 clones were miniprepped, mapped and sequenced to gauge the fidelity of the library, which was then rescued with M13K07 and aliquots of phage stored at −80°C long term.
We tested the similarity of the KRM2 genome-derived probes to other available rhesus sequences to gauge the quality of WGS contigs used in probe design.
This was also used to gauge if a pore had reached its maximal size, with no increase observed throughout the sequence.
The sequence makes sense.
The recent advent of massively parallel RNA sequencing now opens up the possibility to gauge the generality of this observation with a broader phylogenetic sampling.
This enables us to gauge the maximum genomic search space for enhancer hunting: 4 Mb of genomic sequence around the gene of interest (2 Mb on either side).
We gauge the effect of adding prior information to GRISOTTO over 156 well-studied sequence-sets from yeast TF ChiP-chip experiments.
Finally, we have utilized ecological rarefaction in a genomics context to gauge the relative intensity of sampling required for gene discovery (i.e., sequencing depth).
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