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To gauge specificity, interactions were repeated for Leu20Phe FLAG-p7, 15 which conferred resistance to isotopically labeled protein in dye release assays (Fig. 2A).
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A titration of recombinant peptide corresponding to amino acids 20-128 of mouse ANG-1 (Primm, Cambridge, MA) was used to gauge the specificity to mouse ANG-1, and was appropriately detected by the human reagents.
To gauge the specificity of these effects, we also tested cathepsin activity in ML-II embryos that had been rescued by overexpression of WT GNPTAB mRNA (Fig. 3B).
In an attempt to further gauge the specificity of FlAsH-mediated PTP inhibition and its strong dependence on the presence of C333, we asked whether FlAsH could effectively target Shp2 in the presence of free thiols in solution.
As an additional gauge of the specificity of increased cathepsin activity, several glycosidase enzyme activities were measured in 3-dpf WT and ML-II whole embryos.
Host specificity gauges the degree to which a parasite occurs in association with a single host species.
When applied sublingually, OPS provides a sensitivity and specificity for gauging the severity of the distributive defect in sepsis not achieved by conventional monitoring of systemic hemodynamic- or oxygen-derived variables [ 2].
As a result, we cannot determine with certainty to what extent the caretakers reporting of these signs lacks specificity as a gauge of diarrheal severity or goes unrecognized as a trigger for seeking care.
Each antibody was coupled to a unique bead region so that the mix of captors could be analyzed against each biotinylated tracer individually for specificity to be gauged and a first indication of sensitivity to be noted.
The specificity of clinical questions is gauged by explicit descriptions of four dimensions: subjects, interventions, comparators and outcomes of interest.
To systematically gauge the actual trade-off between different levels of sensitivity and specificity in the testing set, the thresholds were set with the testing set.
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