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To gauge relative expression of the corazonin transcript, we normalized levels to those ascertained for RP49.
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Among the 169 GhRabs (Fig. 4), there were 161 genes with log2 FPKM > 1 in at least one of the 16 investigated organs and developmental stages, and these 161 genes were used to gauge the relative expression of each Rab gene.
a Relative expression of myostatin; b relative expression of atrogin-1; c relative expression of MuRF1.
a Relative expression of IGF-1; b relative expression of IGF-1 receptor; c relative expression of MyoD; d relative expression of myogenin.
a AGPP relative expression; b GBSS I relative expression; c GBSS II relative expression; d SSS I relative expression; e SSS II relative expression; f SSS III relative expression; g SBE I relative expression; h SBE IIa relative expression; i SBE IIb relative expression.
Results are shown as relative expression.
A: Relative expression of genes encoding RHD; B: Relative expression of genes encoding C12O; C: Relative expression of genes encoding P34O; D: Relative expression of genes encoding C23O; E: Relative expression of genes encoding G12O.
Figure 5 Relative expression change of miR-26a level.
Results are given as relative expression levels.
Relative expression was calculated using ΔΔCt.
In order to gauge relative replication efficiency, values for ITR2 vectors were divided by the value obtained from the Rep2-ITR2 control.
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