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The 26 stainless steel gauge guide cannula (Plastic One Products, Roanoke, VA, USA) was maintained in place by dental cement anchored to one stainless steel jewelry screw fixed to the skull.
One microlitre of either rAAV-NPY+ or rAAV-empty virus (1×109 Pfu/µl) was injected bilaterally over 10 minutes using a 26 gauge guide cannula and a 33 gauge injector (PlasticsOne, Roanoke, USA) connected to a Hamilton Syringe and a syringe infusion pump (World Precision Instruments, Sarasota, Florida).
Under aseptic conditions, a stereotaxic instrument was used to implant 23 gauge guide cannulae bilaterally into the dorsal hippocampus.
and xylazine (5 mg/kg) and stereotaxically implanted with bilateral 23 gauge guide cannulae targeted at the medial shell of the nucleus accumbens.
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In spring 2004 a 16 gauge model was launched together with an appropriate 15 gauge guiding needle.
Under ketamine and xylazine anesthesia, 24-gauge guide cannulas were implanted bilaterally into the BLA (−1.4 mm anterior to Bregma, ±3.3 mm lateral from the midline, 5.0 mm beneath the surface of the skull).
According to these coordinates a 22-gauge guide cannula (Bilarney / Plastic one, Düsseldorf, Germany) was implanted into the third ventricle.
connected to a 26-gauge guide exteriorized cannula for drug administration in the right or left jugular vein.
The skin was disinfected with 70% isopropyl alcohol and a 22-gauge guide cannula (c313G – Single guide cannula, Plastics One, Inc., Roanoke, VA), cut to a length of 15 mm, was implanted into the right lateral ventricle.
To reduce issues of misalignment of the imaging planes between gold-standard translation stage scans and freehand scans, and to guide subsequent histological sectioning, an 18-gauge guide needle (38 mm length, 840 µm inner diameter) was first inserted into the tissue specimen, and was held in place for the whole duration of OCT scanning to serve as a landmark.
We demonstrated increased rates of pneumothorax and chest tube insertion when 18-gauge guiding needles were used for fiducial marker insertion.
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