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LacZ is a very sensitive reporter gene, and it can be used to gauge expression patterns in vivo, but the animals must be fixed (Fire et al. 1990).
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This latter possibility suggests that, in mice fed a high fat diet, hepatic gene expression patterns can be used to gauge inflammation intensity, and that targeted analytical methods can be developed to exploit this information to gain insight into the tissue remodeling that accompanies excessive intake of dietary fat.
Inflammation profiles are an in silico device that can be used to interpret gene expression patterns, and in the present context, are used to gauge intensity of inflammation in response to HF diet, and to infer which types of leukocytes best explain hepatic gene expression differences between HF-fed and control mice.
Figure 6 mRNA expression patterns.
g Expression patterns of TALE family genes.
OsNLA family expression patterns in rice.
e Expression patterns of C2H2 family genes.
f Expression patterns of HD-ZIP family genes.
b Expression patterns of bHLH family genes.
d Expression patterns of ERF family genes.
c Expression patterns of MYB family genes.
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