Exact(15)
As we show, the introduction of the switch modules enables the creation of sequences of gating events.
Moreover, prevalent intrinsic gating events on the a-HL nanopore were seen only at higher transmembrane potentials as compared to those used in this study (Additional file 1: Figure S5, panel d).
To understand gating events with a time-base many orders-of-magnitude slower than that of atomic motion in voltage-gated ion channels such as the Shaker-type KV channels, a multiscale physical model is constructed from the experimentally well-characterized voltage-sensor (VS) domains coupled to a hydrophobic gate.
Subsequently, 6 and 24 hours later, the number of secreted MVs in respect to the number of cells, was evaluated using flow cytometry by gating events smaller than 1 µm.
All detected gating events were depicted in two histograms.
Our observations of gating events in diffusing ion channels is unprecedented.
Similar(45)
The proper function of even the simplest gates can potentially be essential for catalysis, and the gating event may even represent the rate-limiting step of the catalytic cycle.
40,000 gated events were analysed with CellQuest software (BD).
In all cases, 5×105 to 1×106 gated events were acquired for later analysis.
Analyses were performed using FC500 flow cytometer and CXP software (Beckman Coulter) on at least 1000 gated events.
Samples were fixed with 2% formaldehyde for 1 hr and analyzed using Epics-XL (BeCoulteroulter, Miami, FL) with minimum of 20,000 gated events acquired for each sample.
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