qPCR was performed using Power SYBR Green PCR Master Mix Applied Biosystemss).
Although the requirements for each of these were not fixed in the beginning, comprehensive requirements gathering and analysis operations were performed using brain storming and interviewing methods.
Simulations were performed using PNNL Institutional Computing Resources.
Simulations were performed using Matlab.
This study used snowball sampling [ 4, 8] to identify informants, and data gathering was performed through semi-structured interviews and collection of reports and documents.
Site visits and information gathering were performed from site engineers.
Recruitment and data gathering were performed during January-July, 2009.
The analysis of demographic data and risk factors was performed by gathering the following information: age, gender, primary tumor, bisphosphonate (BP) used, duration of BP intake, history of chemotherapy, use of steroids, and medical history of diabetes.
The pre-test was performed at the end of gathering meeting, after the signing of the informed consent form.
The EURO SENTIMELC study was performed in 2009 and 2010 with data gathering in Belgium, the Netherlands, Italy and Spain (the latter limited to 2010 only).
