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The gathering threshold of a specific Pfam Markov Model is the sequence-based cutoff at which the Pfam curators "gather" sequences to include in that Markov Model.
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In order to gather sequence information on the R. microplus genome structure with a more traditional approach, we also selected five BAC clones which were sequenced to closure.
During the multiple steps of the experimental process there are several opportunities for performing NGS to gather sequence data useful for the purpose of later analysis.
For validation of the successful enrichment of sequences during the experiment and further analysis profiting from the much higher coverage, next generation sequencing was performed to gather sequence data for all screening rounds.
Menus on the JVD interface enable the researcher to gather sequence and annotation data from external databases, and utilize Jalview's own dedicated SOAP web services for sequence alignment with ClustalW (Thompson et al., 1994), Muscle (Edgar, 2004) and MAFFT (Katoh et al., 2005) and secondary structure prediction with Jpred3 (Cole et al., 2008).
We gathered sequences for one or both SAMD9 and SAMD9L genes for species representative of the three clades.
The second cluster gathered sequences of classes I and II from all eukaryotic lineages with the exception of Fungi and most Archaeplastida (Fig. 1 and Additional files 9 and 10).
We gathered sequence data from these transgenes and used it to build a MIR172a precursor map highlighting relevant and neutral positions for its processing.
The procedure gathered sequence information from the expression sequence tag (EST) database and an appropriate algorithm was tailored to extract information from "low quality" sequence data.
GACS gathered sequence information from GenBank, designed and conducted DNA array analysis, and edited the manuscript.
GACS gathered sequence information from GenBank, designed and conducted bioinformatics analysis, and edited the manuscript.
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