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During the multiple steps of the experimental process there are several opportunities for performing NGS to gather sequence data useful for the purpose of later analysis.
In order to gather sequence information on the R. microplus genome structure with a more traditional approach, we also selected five BAC clones which were sequenced to closure.
For validation of the successful enrichment of sequences during the experiment and further analysis profiting from the much higher coverage, next generation sequencing was performed to gather sequence data for all screening rounds.
Menus on the JVD interface enable the researcher to gather sequence and annotation data from external databases, and utilize Jalview's own dedicated SOAP web services for sequence alignment with ClustalW (Thompson et al., 1994), Muscle (Edgar, 2004) and MAFFT (Katoh et al., 2005) and secondary structure prediction with Jpred3 (Cole et al., 2008).
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We gathered sequence data from these transgenes and used it to build a MIR172a precursor map highlighting relevant and neutral positions for its processing.
The procedure gathered sequence information from the expression sequence tag (EST) database and an appropriate algorithm was tailored to extract information from "low quality" sequence data.
GACS gathered sequence information from GenBank, designed and conducted DNA array analysis, and edited the manuscript.
GACS gathered sequence information from GenBank, designed and conducted bioinformatics analysis, and edited the manuscript.
BLW performed molecular methods, gathered sequence information from GenBank and research, conducted bioinformatics analysis, and wrote the manuscript.
ELC carried out molecular methods, gathered sequence information of bats, helped with the analysis of barcode sequences, and edited the manuscript.
We gathered sequences for one or both SAMD9 and SAMD9L genes for species representative of the three clades.
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