Sentence examples for gateway reaction from inspiring English sources
The initial "gateway" reaction in the bacterial metabolism of conjugated bile acids is mediated by bile salt hydrolase [ 33].
These two constructs were combined with a pENTR2-3_t35S and subsequently recombined into the binary destination vector pKGW-RR-MGW by a multisite gateway reaction (Invitrogen, Carlsbad, USA).
Subsequently, both constructs were recombined into pKGWFS7-RR, containing a GUS-GFP fusion reporter, by a single-site gateway reaction (Invitrogen, Carlsbad, USA), creating pKGWFS7-RR_p MtCCD7-GUS and pKGWFS7-RR_p MtCCD8-GUS, respectively.
dac RE -FLP was generated by TOPO cloning the Ring Enhancer (RE) fragment from the regulatory region of dac (Giorgianni and Mann, 2011) into an entry clone followed by a Gateway reaction into a FLP destination plasmid.
Gateway reaction was then performed according to the manufacturer's protocol to generate the expression plasmid pEBTet-HsSAS-6-GFP and pEBTet-HsSAS-6-F131E/R-GFP pEBTet-HsSAS-6-F131E/R-GFP pEBTet-HsSAS-6-F131E/R-GFP pEBTet-HsSAS-6-F131E/R-GFP pEBTet-HsSAS-6-F131E/R-GFP
The pIsl2b Gal4, cmlc2:eGFP construct was generated by a Gateway reaction (MultiSite Gateway Three-Fragment VecThermoFishertion Kit, ThermoFiScientifictific, Waltham, MA) using the p5E-Isl2b (Bet Fredj et al., 2010), pME-Gal4, p3E-pA and the pDest-Tol2 cmlc2:eGFP pDest-Tol2 cmlc207) veGFPrs.
To generate the RNAi vector of APIP12, a 339-bp fragment of at the C-terminus (1,261 and 1,600 bp downstream of the start codon) was amplified and cloned into the pANDA vector (Miki and Shimamoto 2004) by LR gateway reactions.
Gateway reactions were purified using the High Pure 96 UF Cleanup Kit.
Subsequent Gateway reactions were performed to shuffle the sequences into modified pUASTattbCFP and pUASTattbRFP vectors.
Gateway reactions were used to generate the TEM-1 β-lactamase and MyoD fusion proteins flanked by attB sites.
Note that the inhibition of either gateway reactions, v16 (leading to the Shc independent signaling pathway) and v22 (leading to the Shc dependent signaling pathway), do not inhibit ERK activation, but inhibition of both reactions does inhibit ERK activation.
