From St Nikolai, the canal leads to the town walls and a celebrated gateway which frames the port and the Baltic beyond.
The pENTR clones described above were cloned into a pART7-derived plasmid containing the appropriate Gateway cassette in frame with a fluorophore coding region [ 24, 25], using LR Clonase II (Invitrogen®), resulting in pSDM4337 and pSDM4341.
Another figure frames "a gateway" that invites the viewer to "dive in the depth and heights of a timeless horizon, a bridge to endless perspectives".
They serve as a gateway to Harlem and frame the broad lawns of the United Nations.
There, about 15,000 feet above sea level on the China-Pakistan border, glaciered alps frame a gateway labeled in glinting gold: "PEOPLE'S REPUBLIC OF CHINA".
At Wat Pa Phai (or Phapai) on (Sisavangvatthana Road near Sakkarine Road), enter a gateway covered in bougainvillea, framing a novice who is stroking a cat on his lap, with one hand while chanting from a book held in his other.
Reading frame cassette A (Gateway vector conversion system, Invitrogen) was ligated into the linearized vector, giving rise to plasmid vector pUL41-attR-ccdB-cat.
When a PK construct had a low biotin-labeling efficiency and was cleaved near its C-terminus, a new construct was made by fusing the GST nucleotide sequence encoded in the pEU-E01-Gateway-GST vector to the C-terminal codon of the corresponding PK open-reading frame using the Gateway system and the pEU-E01-Gateway-GST vector.
The DsRed2 sequence was removed and replaced by the reading frame A Gateway cassette (Gateway Conversion Kit, Invitrogen), upstream of a stop linker composed of 6 stop codons placed as pairs in the 3 reading frames, resulting in pTrip loxP-Gateway-STOP-loxP-eGFP DeltaU3 destination vector.
Then I see the ethereal dome framed in the gateway that ensures a dramatic entrance and cannot wait to return.
