Sentence examples for gateway expression from inspiring English sources

In a previous study, GFP-tagged genes in Gateway expression vectors were used to examine the sub-cellular localisation of proteins over-expressed by reverse transfection [ 3].

Synthetic sequences of n16N, OC-17 and perlucin with signal peptides are produced in a novel Gateway expression system for Dictyostelium under the control of the [ecmB] promoter.

The vector, which is a modified version of the commercially available expression vector, pET-32a, contains an N-terminal thioredoxin fusion protein and a hexahistidine tag, and is adapted to the Gateway expression system.

The 4164 bp PCR product was crossed into the GATEWAY entry vector pDONR/Zeo using the manufacturer's protocol, resulting in the entry vector pDONR-SHI, and subsequently used to cross into the modified GATEWAY expression vector pET-SHI.

Subsequently, the genes were recombined into gateway expression vectors with an N-terminal 6× myc tag.

A nested PCR approach was used to clone 21 extracellular domains of mouse receptors as CD4 fusions within a mammalian GATEWAY expression vector system.

Failure to secrete is unlikely to be caused by expression from a GATEWAY vector, which requires the translation of the attB2 linker (NPAFLYKVV) as shown in Figure 1, because a comparison of a GATEWAY and non-GATEWAY expression vector showed similar levels of expression for Cd200 EC domain fused to rCD4 (domains 3 + 4) (data not shown).

The mind is our bridge from the subconscious to the conscious, our gateway of expression to the outer world.

To enforce compatibility between entry clones generated by the various laboratories of a scientific community, all our vectors make use of the RfA cassette, which is compatible with the Invitrogen Gateway bacterial expression vectors and Proquest Yeast 2 hybrid system (pDest22 and 32 vectors).

Hence, the construction of pENTR.hU6.hH1 would allow for easy transfer of subcloned shRNA cassettes to different gateway based expression vectors, shRNA targeting of multiple genes, and the increased knockdown of a single target using multiple shRNA sequences.

PHF6, FUBP1, and Smek2 cDNAs were obtained from mRNA isolated from HeLa cells by RT PCR (SuperScript III One-Step RT-PCR system; Life Technologies) and cloned into a His-tag Gateway bacterial expression vector (a gift from Dr. Stephan Geley, University of Innsbruck, Austria).