Sentence examples for gateway entry from inspiring English sources

These cDNAs were in a Gateway entry vector (pENTR221 or pCMV-SPORT6) or cloned into pDONR201 using PCR with KAPA HiFi followed by recombination.

The PCR product was cloned in the pDONR207™ gateway entry vector (Invitrogen™) via BP recombination reaction.

The different primers used to amplify the cDNAs introduced into Gateway entry vector or into pNZ8148 are listed in Table 3.

They were amplified by PCR, cloned into gateway entry vectors, sequenced and recombined into gateway destination vectors to add C-terminal GFP and an upstream UAS sequence.

The amplicons were cloned into pENTR/D-TOPO (Invitrogen) to generate gateway entry constructs, which were fully sequenced to ensure sequence integrity.

AN and SUB cDNA were cloned into gateway entry vector pDONR 201.

This article first describes a cloning strategy based on the NICE system to facilitate cDNA transfer from Gateway entry-vectors into a nisin-inducible vector of L. lactis.

About 250 E. coli clones which were not present in the ASKA library or not successfully ligated from the ASKA library into the Gateway® entry vector were cloned by Gateway® recombinational cloning [ 2].

About 250 E. coli clones which were not present in the ASKA library or which were not successfully cloned from the ASKA library into the Gateway® entry vector were cloned directly by Gateway® recombination (see Methods).

The PCR products were inserted into the Gateway® entry vector pDONR™/Zeo (Invitrogen) by BP-cloning.

We transferred the ASKA library [ 4] into an Gateway® entry vector (pENTR/Zeo) by SfiI restriction enzyme cloning.