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Grey histograms present the FMO (full minus one) signal gated on total monocytes.
For lungs, lower grey histograms present the FMO signal gated on alveolar macrophages (AM) (pink gate).
GFPlo and GFPhi T cells were then gated on Thy1.1 reporter expression as indicated.
Left panels are pre-gated on CD45.1+CD45.2− cells and right three panels are further gated on TCR Vα2+ OTII cells.
MHCII gated on FITC- cells.
FoxP3+ gated on CD4+ cells.
Dot plots are gated on CD8− lymphocytes.
Dot plots are gated on CD4− lymphocytes.
For FACS, we first gated on single cells and then gated on the enriched monocytes population.
F4-80 gated on CD11c+ cells, CD11b Vs.
(c) Expression of LysM-GFP in brain monocytes (gated on LiveDead− CD45.2hi CD11b+ Thy1.2− Ly6C+ Ly6G−) and microglia (gated on LiveDead− CD45.2low CD45.2lowy1.2− Ly6CD45.2low.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com