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FSC-A/SSC-A were used to exclude debris and apoptotic cells; doublets were excluded by using FSC-W/FSC-H; the EYFP + sorting gate was set using SSC-A/FITC-A (Supplementary Fig. 1i).
Debris and apoptotic cells were excluded by using FSC-A/SSC-A; singlets were identified by using FSC-H/FSC-A and SSC-W/SSC-H; the EYFP + analysis gate was set using SSC-A/YFP-A (Supplementary Fig. 1h,i).
The time lag to the first gate was set at 1200 us, which means that the range was extended from 180 to 3555 km for one beam.
An acquisition gate was set to include ~20,000 of the centrally located cells for each sample acquisition using linear forward scatter versus linear side scatter.
During the period of our interest, the frequency was around 11 MHz, and the first range gate was set to 180 km with a range resolution of 45 km.
The gate was set to exclude approximately 99.5% of the negative control cells.
Similar(18)
The side input of this OR gate is set to 0 to simulate a fault-free site, or set to 1 to inject the s-a-1 fault.
Gating was set using control samples without primary antibody.
Gating was set based on the isotype-staining profiles.
A gating was set to exclude apoptotic and dead cells.
Gating was set on side-scatter, then by the fluorescence measured on a linear scale.
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