(D ) Circadian gating of light signalling pathway, adapted from (Iyer et al., 2014).
Notably, the PRR9 expression level was not maximal compared to peak levels, suggesting that another factor is involved in the gating of light responses in the dark.
The expected effect of the VVD protein in modulating the circadian gating of light responses may in part account for these discrepancies.
ELF3 acts in the gating of light input to the core oscillator and is necessary for light induced expression of CCA1/ LHY; as such, ELF3 contributes to the resetting of the clock each day [ 33, 34, 32].
The third N. crassa photoreceptor, VIVID (VVD) is responsible for gating of light responses [ 16], acts negatively on the White collar complex (WCC) and can act as a universal brake on light response [ 17- 21].
Through its function as a regulator of the WCC, VVD also plays roles in entrainment of the clock, including gating of light input and temperature compensation of phase (Elvin et al. 2005; Heintzen et al. 2001; Hunt et al. 2007).
(B ) Circadian gating of light-modulated transcripts.
Relatively effective fluorescence detection has been demonstrated numerous times using microscope optics or similar setups [ 2– 4], however little attention has been paid to the important gating parameters of light scattering, and even less attention has been paid to integrating such optical systems.
RBCs were gated on light scatter characteristics, and a total of at least 30,000 events in gating were collected for each sample.
THP-1 live cells were gated on light scatter characteristics, and a total of at least 30,000 events in gating were collected for each sample.
