Sentence examples for gate assembly from inspiring English sources

Golden Gate assembly was used to assemble the constructed vectors into the all-in-one CRISPR/Cas9 vectors.

The donor transcription units were amplified with primers that introduced terminal BsaI restriction sites, which were subsequently assembled into the universal vector pYW0120 by Golden Gate assembly.

The donor fragments are generated from a universal vector, which is an E. coli-based plasmid enabled for yeast Golden Gate assembly and red/white E. coli colony screening23.

The limiting factor in many pulsed systems is often the capacitance of the gate assembly.

Here, the gate assembly was mounted approximately 100 μm adjacent to the CNT electron emitter resulting in a device geometry capable of sustaining a beam current of several milliamps, although this would likely prove challenging to manufacture in a practical X-ray source.

We generated two pairs of DNA binding domains for targeted sites, Tyr I and Tyr II, in the first exon of the tyrosinase gene of Xenopus tropicalis (X. tropicalis) using the Golden Gate assembly method (Cermak et al., 2011).

Compared with other connexins, Cx43 has a larger intracellular C-terminal tail, and (de phosphorylation of serines in this domain represents an important regulatory mechanism for GJ gating, assembly, trafficking, and degradation.

Assemble the TALENs using the Golden Gate TALEN assembly kit [ 20, 23 ]; the details of this method won't be discussed here.

The total thickness of the complete gate valve assembly is 1.03 in.

Based on the simulation results, the flow and heat transfer characteristics of the underwater gate valve assembly are discussed.

The conjugate heat transfer of an underwater gate valve assembly is numerically investigated by 3D steady RANS CFD approach.