The GAS PCR assay appears to be an accurate and rapid molecular assay for GAS detection in children and adolescents with pharyngitis.
Since the GAS PCR assay, including DNA extraction, can be performed in approximately 1 hour, prospective studies of this assay are warranted to evaluate the clinical impact of the assay on management of patients with pharyngitis.
Since the GAS PCR assay, including DNA extraction, can be performed in approximately 1 hour, prospective studies of this assay are warranted to evaluate the clinical impact of the assay on the management of patients with pharyngitis.
For accurate determination of PepP activity, a novel gas chromatographic assay was established.
Furthermore, we expressed lactococcal PepP for the first time in E. coli, and following automated purification, we characterized lactococcal PepP and developed a new gas chromatographic assay for accurate PepP enzyme activity determination and a novel specific activity staining method for PepP for use in native-PAGE analysis.
The GAS PCR assay may be useful for clinical laboratories.
The rapid internally-controlled GAS PCR assay described performed as well as the currently accepted standard method of conventional swab culture for GAS detection.
The GAS PCR assay also demonstrates for the first time the clinical usefulness of flocked swabs in liquid transport media for bacterial molecular assays.
We compared the GAS PCR assay to GAS culture results using a collection of archived throat swab samples obtained during a study comparing the performance of conventional and flocked throat swabs.
We performed a study of a laboratory-developed internally-controlled rapid Group A streptococcus (GAS) PCR assay using flocked swab throat specimens.
To further investigate the changes of different fatty acids in diabetic serum and hearts, we observed 14 different types of FFA by capillary gas chromatography assay (Table 1).
