PCNA acts as a DNA replication clamp that is involved in numerous aspects of DNA replication and can also be involved in both generating gapped substrates and assembling DNA replication complexes on them (Andersen et al., 2008; Caldecott, 2008).
An opening gap is for assembling and disassembling cast and makes the cast adjustable during the treatment of an injured limb.
Later on, manual analysis of the graph of alternative contig connections produced by Newbler was performed with an in-house software, in order to close gaps in mitochondrial contigs and assemble long repetitive regions.
On the basis of immunofluorescent labelling of keratin-14 and Cx43, mammary epithelium cultures were deemed highly enriched in myoepithelial cells and assembled gap junctions.
The gap-filled scaffolds were clustered and assembled to get sequences with least Ns and cannot be extended on either end.
Thus, the Germany pool had fewer gaps (from no gaps in SILV assembled clones to 82 gaps in KITL assembled clones) in the sequences (Table 1) and the sum of the contigs from clones for individual genes (one single or two overlaping clones per locus) averaged approximately 240 kb.
This study begins to address this gap by assembling a library of mitochondrial COI sequences, or DNA barcodes, for Argentinian birds and comparing their patterns of genetic diversity to those of North American birds.
While school officials decided to postpone planning a memorial service until the families had made funeral arrangements, the teenagers filled the gap, assembling makeshift memorials and holding a service on the baseball field, where about 200 of the 1,200 students gathered after school.
The modification of gaps in assembled sequences affects the positions in the reads the assemblies are composed of.
Additional optional steps include 7) final gap closing, 8) assembling single-end sequence reads, and 9) additional ways to access the VirAmp pipeline.
