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Fifty-three physical gaps, including novel gaps identified in this study, remained in the genome assembly (Additional file 2); note, one gap was removed by visual inspection of GS-FLX reads (see "Detection of misassembling and erroneous large gaps").
In one case, a physical gap that had originally been inserted into the reference was found to be unnecessary based on our long read validation, and this gap was removed.
Allelic amino acid sequences were aligned and each site containing a gap was removed from the alignment (indel sites are illustrated in Additional file 1).
HMMsearch introduced a gap of length 7 between the next-to-last (R52) and the last (Q53) residue in the sequence; in this case, the gap was removed, placing R52 directly next to Q53, thereby producing a better-quality alignment.
Similar(56)
Ambiguous regions and all sites including a gap were removed from the alignment.
Additionally, the sequences with the region of gap were removed.
Columns in which the seed had a gap were removed.
Alignment positions for which a fraction > 0.95 of the individuals have a gap are removed from the alignment.
Afterwards, all positions containing a gap were removed from the alignment and the amino acids were replaced by their corresponding codons.
In contrast, when the interaction of GDIs with GAPs was removed, Rho activation level decreased and was not sustained.
Any mPing candidate loci containing sequence gaps were removed from the analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com