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An evaluation of the Health Nutrition and Population Sector Program by the government of Bangladesh (GoB) raised quality issues as priority gap to resolve in service provision in healthcare delivery, particularly in MNH.
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A total of 4392 primer walk reactions, 5 transposon bombs, 28 clone shatter libraries, and 4 PCR shatter libraries were necessary to close gaps, to resolve repetitive regions, and to raise the quality of the finished sequence.
A total of 952 primer walk reactions and 3 transposon bombs were necessary to close gaps, to resolve repetitive regions, and to raise the quality of the finished sequence.
After about four iterative cycles of the above "finish" procedures to close gaps and to resolve the low-quality regions, the PCR product obtained by using total genomic DNA as template was sequenced from both ends to close the last physical gap.
Dr. Shubin said Tiktaalik was "still on the fish end of things, but it neatly fills a morphological gap and helps to resolve the relative timing of this complex transition".
The equivalent single-layer approach and Mindlin-Reissner kinematics are used in the element formulation together with the discrete shear gap (DSG) technique to resolve the shear locking and strain smoothing technique to improve the performance.
Instead, lawmakers are staring down budget gaps as they try to resolve a historic strike that has shuttered schools in all 55 counties and galvanized austerity-weary residents.
These may contain misassemblies or require further 'finishing' work to resolve gaps (Chain et al., 2009).
Genomes were finished by using PCR and traditional dideoxynucleotide sequencing (Sanger) to resolve gaps and repetitive and low coverage areas.
Sanger sequencing of PCR products and local reassembly were used to resolve gaps, misassemblies and unclear regions.
Paired-end reads that were used to resolve gaps were verified by checking the position and orientation of their downstream mate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com