The political gap spans not one generation, but three.
At one stage I turned a corner at high speed to find a huge gap, spanned by a painfully thin tree trunk.
This problem becomes especially acute when the time gap spans millennia and a number of sister languages become extinct, events that obscure possible intermediate links in reconstruction.
Instead, D. willistoni shows a gap spanning the EFsec locus.
Gaps between contigs were closed by custom primer walks on gap spanning clones or PCR products.
Gaps between contigs were closed with gap spanning primers, designed with Primer3 [ 62].
A majority of contigs were ordered and oriented by read pairs of gap spanning subclones.
For VIP this gap spanned between 11% and 31%, with 95% confidence intervals that did not overlap.
In theory, paired-end mapping should mitigate this problem if the gap spanned by the paired reads is larger than the repeat itself.
Regions of lower quality data were sequenced by direct PCR on genomic DNA, or additional sequencing of select gap spanning clones.
