We therefore used only the conventional form of the assay, in which a 1 kb p53 PCR product is gap repaired into the pRDI-22 yeast expression vector (Waridel et al, 1997).
Kovtun, I. V. & McMurray, C. T. Trinucleotide expansion in haploid germ cells by gap repair.
Two UV-sensitive chromosomal mutant alleles of RAD3, rad3-1 and rad3-2, werescuedued by gap repair of a centromeric plasmid, and their sequences were determined.
Gerami-Nejad, M., Zacchi, L. F., McClellan, M., Matter, K. & Berman, J. Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans.
Nassif, N., Penney, J., Pal, S., Engels, W. R. & Gloor, G. B. Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair.
Mr. Booker combined his familiar themes of unity with specific policies to close the racial wealth gap, repair what he considers a broken criminal justice system and set the country on a path to be a leader in climate change.
Plasmids were constructed by gap repair in yeast (Oldenburg et al. 1997), using yeast strain CEN.PK 113-5D (MATa MAL2-8 c SUC2 urand52), anamedmed according to Table 2.
The gene was cloned in yeast vector by a gap repair strategy.
Subsequent strand separation and gap repair lead to the presence of short direct repeats flanking the proviral DNA [15], [16].
The fragments were excised from the plasmid using flanking restriction sites and used in gap repair experiments with plasmid pJV362.
