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This study contributes to a current gap of expression of participants׳ gains, especially from an indigenous and marginalized rural communities׳ perspective.
As a matter of fact, it is not possible to design a multiplex of Taqman including the housekeeping gene, β-Actin, and the RRs genes together because the gap of expression between them is so wide that the amplification of β-Actin will be inhibited when RRs become detectable for the same qPCR cDNA inputs.
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Clear gaps of weak expression could be found in the forebrain midbrain boundary from the 9-somite stage to 24 hpf (Fig. 2C E).
The formation of borders of gap gene expression domains could be understood in terms of three qualitative dynamical mechanisms: (1) The movement of attractors; (2) Selection of attractors; (3) Selection of states on a one dimensional attracting manifold.
Even though our analysis of combinations of gap gene expression indicated that there were no new cell fates, we observed significant differences in expression of two gap gene targets: eve and ftz (Fig. 6).
This analysis showed that the observed reduction in variation of gap gene expression patterns is a consequence of the action of robust attracting states [ 4].
The second mechanism involves regulation of the boundaries of gap gene expression by gap gene cross-regulation [28], [29], which become less coupled to Bcd over time [30], as previously proposed.
In [ 3, 4], we used the "gene circuit" approach to demonstrate that the reduction in variance of gap gene expression was a consequence of gap gene cross regulation.
However, the precise dynamics of gap gene expression and the nature of gap gene cross-regulation have not yet been investigated in this species.
Given the fact that the models correctly predict the basic features of gap gene expression in the majority of the constructs we conclude that all the model correctly account for the functional role of most of the included CRM's binding sites.
If the abnormalities in segmentation seen in dicer-2 embryos exposed to the temperature step are due to an inability to correct for difference in developmental rates between the two halves of the embryo, we expected to observe a normal anterior domain of gap gene expression, and an underdeveloped or missing posterior domain of gap gene expression.
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