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Unless using excessive gap insertion, both landmark features could not be homologized across the alignment, as the Rickettsia protein has an extended sequence between the Cys residue and the P[ILV]NK motif.
However, based on a gap insertion:substitution cost ratio 10 1, primary homologies could be unambiguously established.
The thresholds for gap insertion and for gap deletion in the simulations by Rose were set to 0.0001.
We found that this limited set of gap insertion effectively helps to capture composite motifs with multiple functional elements.
This technique discourages multiple and disjoined gap insertion blocks unless their inclusion greatly improves the pair-wise alignment score.
PRANK+F is a novel attempt to model the gap insertion required by the alignment process in a phylogenetically meaningful way.
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The two sets of pseudomolecules differed slightly due to differences in selection of the clones underlying the MTP and the lengths of gap insertions used by the two projects.
In contrast, the dog and rodent sequences contained multiple nucleotide insertions and /or deletions, resulting in multiple gap insertions throughout the alignment (Figure S1).
We allow for gaps in the maps by assigning a gap penalty that penalizes gap insertions between highly interacting residues.
Although there are many different ways to insert gaps in the 8-mer words, we consider only 8 specific patterns of gap insertions (Fig. 1F).
In order to ensure that clusters of interacting residues are not split, we assign a high penalty for gap insertions in rows and columns containing a high number of interactions.
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