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The default gap opening and gap extension penalties were used.
The gap opening (w0) and gap extension (w1) penalties are applied to other regions.
The default gap opening and gap extension penalties were used (15.0 and 6.66).
A penalty of −2 for a Gap Extension and −8 for a Gap Opening.
Pairwise and multiple alignments were performed using BLOSUM protein weight series matrices, with default gap opening and gap extension penalties.
Multiple sequence alignment was done using Clustalw (http://www.ebi.ac.uk/Tools/clustalw2/, gap opening penalty = 10 and gap extension penalty = 0.05).
Blastn parameters for match/mismatch score, gap open and gap extension cost were left at their default settings.
The default parameters for gap open penalty, gap extension penalty, and perform fft were used (1.53, 0.12, "localpair").
I used combinations of gap opening penalties of 5, 4, 3, 2, and 1 with gap extension penalties of 2 and 1.
Finally, a gap opening penalty of 10 and a uniform gap extension penalty of 0.2 were chosen based on the alignment scores.
Protein weight matrix Gonnet, with Gap Opening 10 and Gap Extension 0.2 was used for multiple alignments that were manually refined in GeneDoc v.2.7.0 [34].
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