It is his awareness of the status gap, duplicated in his own subordinate position in the office, and his male pride, that prevent him from doing anything about the awful longing in his heart.
Hence, the results of Rasch model analysis provided a comprehensive basis for researchers to revise or develop the questionnaire, and highlighted aspects for improvement, such as gap, and duplicate items.
Work hard to close the gap and to duplicate the luminary's points of superiority over the field.
The segments should contain at least two pairs of duplicates, and the gap size between two neighbouring duplicates in either segment should be less than 30 genes [ 13].
The sequences were aligned using ClustalX (ver. 1.81; [35]) and then checked for duplicates, gaps, ambiguous bases, pseudogenes and sequences which were shorter than the required 610 bases starting with 5'GCTATAATAACT.
Cluster analysis was performed using the duplications, substitutions, indels (DSI) algorithm in BioNumerics with the following parameters: 250 % gap creation cost; 50 % gap extension, duplicate creation and duplicate extension costs; maximum three repeat duplication; and 1.00 % bin grouping distance.
Each cDNA probe was printed in duplicate onto ULTRA gaps II coated slides (Corning Inc., NY, USA) using a Qarray2 (Genetix Ltd ,Queensway, UK) with a telechem printing head and 16 split pins (Biorobotics, Cambridge, UK), and with a 4 x 4 configuration.
Unlike bacteria, in eukaryotes there is a definite time gap between when the genome is duplicated and when it is physically separated.
In all five of these cases, we found a gap at the proposed position where the duplicate locus should have been located in the assembly.
Examination of the zebrafish genome revealed a small number of loci containing TFRs that appear to be duplicated and often separated by a gap in the genome assembly.
