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Subsequently, we developed a real-time PCR (TaqMan assay) that specifically detects the swCt variant by using a probe that spans the 377-bp left and right gap border sequences: probe- swCT 5′- FAM GGA TCC GTT TGT TCT GG MGB -3′.
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His compositions tend toward verticality, a gap bordered by black or, as he puts it: "A curtain you can look through into this mental landscape". The tall space in the middle is populated with clamoring shapes and screaming faces, the monsters ever present, diminished in size though not in number.
MDA-MB-231 cells were allowed to migrate over a cell-free gap (bordered by the pair of black lines) after treated with PEI-GO:siCXCR4 complexes of mass ratios 0.3:1 (d), 0.5 1 (e), and 1 1 (f) for 48 h.
This was supported by synteny analysis, which indicated that the initial assembly covered all genes present within this region in sequenced fish genomes, and by conducting a BLAST search of gap ends, which revealed that many of the gaps bordered known salmonid repetitive elements [ 10].
Border Patrol agents cannot hear smugglers digging and do not know how many tunnels there are, a gap in border security that homeland security experts say renders talk of a wall moot.
But no technology exists to reliably detect the tunnels, and experts say it may be years before such a system is developed....Border Patrol agents cannot hear smugglers digging and do not know how many tunnels there are, a gap in border security that homeland security experts say renders talk of a wall moot.
Despite numerous attempts, even when utilizing an additional male AU9 BAC library, the gap between border A and HSY remained unfilled.
The main goal was to decode the mechanistic basis of the gap gene border formation and stability under the Bcd morphogen variance.
The physical map of MSY and HSY contains a gap at the border 'A' region (border 'A' represents the left border and border 'B' represents the right border in each chromosome) [ 30, 39].
He said that the Palestinians had been prepared to go to Washington in early January 2009 to try to bridge the gaps on border issues, but that Israel had refused because it was in the midst of a three-week military campaign in Gaza.
The resulting model, when run with Bcd gradients from many individual embryos, correctly predicted the variance in position of six gap gene borders.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com