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As FANCL directly interacts with PCNA via its central DRWD domain (Geng et al. 2010), these data suggest that FANCL is a genuine reader of ubiquitylated PCNA that gains its activity by recognition of the modification.
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The ATPase activity gains its highest sensitivity towards catalase at 10 min of reoxygenation attaining a value similar to that of 5 min of hypoxic perfusion.
However, to gain its full activity several events must be met: intracellular alkalinization, presence of progesterone and membrane depolarization (Ren et al., 2001; Kirichok et al., 2006; Lishko and Kirichok, 2010; Lishko et al., 2011; Strunker et al., 2011).
Tamoxifen was used to induce Cre activity in gaining its access to the nuclear compartment from the cytoplasm.
The tetR gene product on its own is inactive, and it is only after the addition of doxycycline (Dox) that it gains activity as a transcription factor by binding and activating the TRE promoter region and transcribing the adjoining gene, in this case Cre [40].
Increasing DOPE often gains high activity for the HCII structure promoted by DOPE.
We find that purified USP13 indeed gains some activity at pH 8.0 compared to pH 7.4.
Our in vivo data here confirm that the ecdysone pathway indeed gains in activity when Smr is mutated.
It is currently gaining in activity and is expected to peak in 2013 or 2014, although nobody can be sure.
In contrast, endomorphin-1 gained activity with Y328F but lost activity at W320A, in both pathways.
It was therefore reasonable to ascertain whether the sub-cellular distribution of chicken Mx was an important determinant in its antiviral activity (or lack thereof), and whether an otherwise inactive protein might gain antiviral activity upon nuclear re-localisation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com