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Controls (binding buffer only, PI only, and annexin-V only) were used to set appropriate detector gains, compensation, and quadrant gates in the FACSCalibur flow cytometer.
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Instrument setting parameters (gains, compensations, and threshold) were set with machine software (CXP Software Beckman Coulterr) in conjunction with calibration beads (Flow-set beads, Cytocomp kit, and CYTO-TROL Control Cells).
Finally, gain compensation and multi-band blending are optionally used as offline steps to compensate for photometric defects and seams caused by misregistrations.
Most are pranks by children seeking attention; some are murkier attempts by parents to gain compensation.
It took an act of Congress and a treaty with Mexico to gain compensation for their merchandise.
"North Koreans must understand that they will not be able to gain compensation by provoking a crisis," Mr. Lee said.
The incident happened in December last year but has hit the headlines as the couple continue their efforts to gain compensation.
Modern technology lets you use various tools (such as audio compression and gain compensation) to make sure every song on an album sounds absolutely as loud as possible.
Has gone close twice since stepping up to seven furlongs and can gain compensation for a luckless run here earlier in the month.
She knew only too well that asbestos-related deaths are still rising and that gaining compensation remains as fraught as ever.
If the court somehow contrives a "crime without a criminal" so as to gain compensation, the impact on thousands of distressed mothers will surely be appalling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com