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In this study, we used T = 0.15 as the absolute value for the threshold, where gains are defined by copy number level > T and losses by copy number levels <− T. To systematically look for subtype-specific genomic events, we developed a method called Subtype-Enriched Alterations (SEA).
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Gains were defined as a log2ratio ≥0.58 and homozygous losses as a log2ratio ≤−0.60.
Gains were defined as at least two neighbouring oligonucleotides with deviations of 0.2 or more from log2 ratio = 0.0.
Polysomies (chromosomal gains) were defined as more than 10% of nuclei containing three or more CEP signals.
The cutoff values for losses and gains were defined using a panel of 10 paraffin-embedded control sections of non-neoplastic tissues and were calculated as 10%% for gain and 12 % for loss.
Signals corresponding to both the RP11-165M24 and the chromosome 17 centromeric probes were scored in 200 non-overlapping nuclei of the tumoral zone and gains were defined in samples where ≥ 10% of the analyzed nuclei presented a ratio between the RP11-165M24 and the reference probe ≥ 1.5.
Unlike linear channels, the SER and the diversity gain are defined in terms of PSNR in peak-power-limited channels.
The diversity order and coding gain are defined as[15] G d = lim γ ̄ → ∞ log P b γ ̄ log γ ̄ (14a).
Firstly, recall that the diversity and multiplexing gain are defined as [7] d ≜ − lim ρ → ∞ log [ P e ] log ρ and r ≜ lim ρ → ∞ R log ρ, (4).
The horizontal antenna gain and vertical antenna gain are defined as A H = − min 12 ( φ − φ as φ 3 dB ) 2, A m (3) A V = − min 12 ( θ − θ gtilt θ 3 dB ) 2, SLA v. (4).
The working criteria for loss or gain are defined as the chromosomal region with at least four consecutive SNPs with CN < 1.6, or at least four consecutive SNPs with CN > 3.5, respectively.
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